سنتز نانوالیاف بارگزاری شده با اسانس سرو نقره ای Cupressus arizonica Greene و ارزیابی آن روی بید سیب‌زمینی Phthorimaea operculella (Lepidoptera: Gelechiidae)

The potato tuber moth (PTM), Phthorimaea operculella (Zeller), is a major pest of potato, both in the field and storehouses. In this study, we have evaluated the lethal effects and persistence of Cupressus arizonica Greene pure (PEO) and nano-formulated essential oil (NFO) on different developmental stages (egg, male and female adults) of potato tuber moth. Essential oil was extracted by hydro-distillation using a Clevenger-type apparatus. The essential oil was analyzed by gas chromatography/mass spectrometry (GC/MS). Nanofibers were produced by electrospinning technique. The morphology of nanofibers was investigated by SEM. Fourier transform infrared (FTIR) was used to identify the characteristic functional groups in the PEO, nanofiber and PEO/NFO scaffold. Bioassays were performed in 250 ml glass jars. The essential oil consisted of l- Limonene as the most abundant component (7.75%), followed by Epi-bicyclosesquiphellandrene (7.23%) and α- Pinene (6.79%). The results of FTIR spectra showed the establishment of the functional groups of PEO on the structure of the nanofiber. The images of SEM also demonstrated the establishment of PEO in the structure of the nanofiber. LC50 values of PEO and NFO were estimated 109.36 and 49.24 µl/l air for eggs, 17.88 and 8 µl/l air for female adults, and 16.4 and 6.8 µl/l air for male adults, respectively. Persistence data showed that nano-formulated essential oil (51 days) in comparison with pure essential oil (17 day) had longer persistence. The results demonstrated that C. arizonica PEO and its nano-formulation could play an important role as natural pesticides for the management of potato tuber moth.