بررسی تنوع ژنتیکی Eurygaster integriceps در استان‌های مختلف ایران

(Eurygaster integriceps Put.; Het.: Scutelleridae) has long been considered a significant threat to regional economies and food security. The impact of this pest on the wheat crop has led to extensive research on its biology, ecology and management strategies. Understanding the genetic diversity of this species is important for developing targeted control measures and conservation efforts. In this study, the genetic diversity of populations of this pest from different provinces was investigated by comparingthe cytochrome oxidase I gene sequence. The genome of each adult insect was extracted using the recipe of Sina PureTM DNA kit and amplified by PCR reaction using primer pairs. Initial alignment of edited sequences was done with ClustalW software. In the next step, the sequences were manually edited in BioEdit software. The sequenced fragments were compared with the nucleotide sequence extracted from the gene bank of the National Center for Genetic Information (NCBI) and the phylogenic tree was drawn using the neighbor-joining method The genetic parameters related to seven haplotypes in these populations, which can contribute to the flexibility of the species against environmental changes and selective pressures, were investigated. Haplotic diversity and nucleotide diversity in all populations were calculated as 0.825 and 0.00389, respectively Comparing the sequences showed the presence of five polymorphic loci among the studied loci. The results of geographical grouping showed that the northern regions of the country have more haplotype diversity than other regions. This phenomenon can be attributed to the combined effects, increased availability of resources, variable selection pressures, facilitation of gene flow and other factors, which contribute
to the proliferation of haplotypes