واکنش ارقام گندم نان به نماتد سیستی غلات Heterodera filipjevi و بررسی فعالیت های ژنی و آنزیمی

Cereal-cyst-nematode-(CCN), Heterodera filipjevi, causes a serious economic threat to Wheat production worldwide. The screening of wheat genotypes for characterization of resistance against CCN is a critical step to develop resistant cultivars. Thus, in this study, the reaction wheat cultivars, to H. filipjevi was investigated, and also, genetic variability of the germplasm, using simple sequence repeat (SSR) primer pairs was analyzed. The changes in the expression of markers gene, Cre3, PRX-groups (TaPrx111 and TaPrx112), were analyzed using qRT-PCR at various time intervals. In addition, the activities of defense-related enzymes; peroxidase and catalase were analyzed. RNA was extracted from roots 4 and 7 days after inoculation (dpi) and from leaves 6 and 7 weeks after inoculation (wpi). The cluster diagram divided the wheat genotypes into four groups i.e., resistant, partially resistant, susceptible and highly susceptible accordingly, among the cultivars, Bam and Behrang were included in the resistant group. This diagram was consistent with the results of genetic variation with SSR markers. Expression of Cre3, peroxidases-(PRX)-TaPrx111 and TaPrx112-genes increased at 4 dpi by 6.32, 20 and 8.71-fold in the roots, respectively. However, at 7-dpi, the expression of Cre3, TaPrx111 and TaPrx112-genes increased by-15.98, 5.93, and 16.59, respectively. Similarly, an increasing trend was recorded in leaves. Our findings demonstrated that the activity of -POX and CAT increased by-1.45 to 2.54 times with CCN infected in resistance-genotypes. Surprisingly, PRX-gene-marker, basically resistant to abiotic-stresses such as high-salinity and drought, and also regulating the ROS-balance in plants, similarly modulates-immunity against-H. filipjevi. Conclusively, the accessions used in this-study that exhibited resistance-and-moderate-resistance offer novel resistance sourcing for wheat-breeding-programs.