اولین گزارش از آلودگی لیسیانتوس (Eustoma grandiflorum) به ویروس پیچیدگی برگ زرد گوجه‌فرنگی در ایران

Iran. In 1400-1401, virus-like symptoms, including leaf curling, swelling of veins on the lower surface of the leaves, and stunting, were observed on lisianthus plants grown at different locations in Pakdasht (Golzar Paeen and Filestan), Iran. Plants infected at an early stage did not yield flowers, but late infections did not impair flowering. Tomato yellow leaf curl virus is a geminivirus, transmitted by whiteflies (Bemisia tabaci) and causes destructive disease on several crops. This virus has a wide host range and has been reported from ornamental plants. Considering the similarity of the observed symptoms to the symptoms reported for this virus, as well as the contamination of the greenhouses infected with whitefly, to assess the presence or absence of the virus, DNA extraction from samples with viral symptoms and without symptoms was carried out by the CTAB method. The expected fragments with a size of about 250 and 670 bp were obtained following polymerase chain reaction (PCR) based on two specific pairs of primers (TYLCV-sense and TYLCV-antisense) synthesis part of coat protein gene, and (TYLCV-MP-R and TYLCV-MP-F) synthesis part of movement protein gene, and electrophoresis of products on 1% agarose gel. In this research, specific 18S rDNA primers (18s 1 and 18s 2) were used as internal control, and TYLCV-Almeria isolate was used as a positive control for assessing the presence of the virus in plants with and without symptoms. Sequencing analyses showed the similarity 99 % of the amplified fragment sequences with a novel isolate of tomato yellow leaf curl virus infecting zucchini in Pakistan. This is the first report of the occurrence of tomato yellow leaf curl virus in lisianthus in Iran.