آنالیز پروموتور ژن پروتئین شوک حرارتی (HSP70) در مقاومت گیاه آرابیدوپسیس به بیمارگرهای گیاهی

Heat shock protein (HSP70) is one of the important groups of chaperone molecules in eukaryotic cells that plays a role in plant defense responses against pathogens. This study aimed to determine the molecular mechanisms related to the HSP70 gene involved in plant defense against pathogens. In this regard, the bioinformatic analysis of heat shock protein (HSP70) promoter was conducted in Arabidopsis thaliana model plant. For this purpose, the sequence of 2000 bp promoter regions (upstream of the transcription start site) was extracted from the TAIR database. Bioinformatic analysis of the promoter was conducted using Plant CARE and PlantPAN databases. The cis-acting regulatory elements and their respective transcription factors (TFs) were identified in the promoter of HSP70. Chromosome map of the HSP70 gene was obtained by TAIR. The results showed that the HSP70 gene is located on chromosome number three in Arabidopsis. Also, transcription factors DOF, GATA; tify and AP2; ERF showed the highest number of transcription factors binding to the HSP70 gene promoter with the numbers of 220, 163 and 141, respectively. In addition, TATA-box and CAAT-box showed the most regulatory elements with 17 and 10 respectively. Moreover, HOP3, HSFA2, HSP90-5, HSP90-1, CPN60, CLPB1 and SGT1B revealed the most interaction with HSP70 protein. The results of this study showed that the transcription factors and their binding sites on the HSP70 promoter, as well as regulatory elements on the promoter with the role of resistance to plant pathogens may play important roles in regulating gene expression and defense responses against plant pathogens.