اثر نانوذارت اکسید‌آهن سنتز شده با عصاره تلخه و چای سبز بر پاتووا‌رهای عامل لکه نواری باکتریایی گندم

Cereals, especially wheat, are among the strategic and important agricultural products. Bacterial leaf streak (BTS) of wheat is considered one of the most destructive diseases of cereals. Due to the fact that the use of chemical poisons is prohibited and obsolete due to their widespread toxicity on non-target organisms and the resistance of plant pathogenic bacteria to many of these poisons. Finding suitable alternative methods to control plant diseases is an obvious need. In this research, in order to investigate the antibacterial effect of iron oxide nanoparticles synthesized using two extracts of Acroptilon repens and Camellia sinensis extract that cause on bacterial leaf streak pathovars of wheat, an experiment was conducted in the form of a completely randomized design with broth microdilution method in three replicates. Infrared spectroscopic analysis, scanning electron microscope and X-ray diffraction were used to identify the synthesized nanoparticles. The color change in the solution and the settling of brown sediments at the bottom of the vessel confirmed the successful green synthesis of iron oxide nanoparticles. In the spectroscopic analysis, the presence of a peak in the range of 490 to 600 nm indicates the biosynthesis of these nanoparticles with extracts of Acroptilon repens and Camellia sinensis. The size and morphology of nanoparticles synthesized by biological method was determined by scanning electron microscope and it was found that the shape of the particles is polyhedral and round, their average size in nanoparticles synthesized with Acroptilon repens extract is about 40 to 60 nm and in nanoparticles synthesized with Camellia sinensis extract 50 It is up to 80 nm. Also, the results showed that iron oxide nanoparticles synthesized using Acroptilon repens extract inhibit the pathovar Xanthomonas translucens pv. Cerealis at a concentration of 512 mg/ml and inhibiting the pathovars Xanthomonas translucens pv. Undulosa and Xanthomonas translucens pv. translucens at a concentration of 256 mg/ml had an inhibitory effect, and the studied variables showed that the type of bacterial pathovar, the contact time and the size of iron oxide nanoparticles are among the factors influencing the antimicrobial effect of iron oxide nanoparticles against bacterial pathovars that cause Bacterial leaf streak of wheat.