اولین گزارش از Acrophialophora teleoafricana به عنوان قارچ اندوفیت لوبیا در دنیا

Endophytic fungi live in plant tissues without any symptoms and have beneficial effects on the host in various plant tissues, exhibiting a wide range of diversity and distribution. Fungal endophytes have significant environmental and biological effects on plant communities worldwide and are considered one of the most important and effective factors in controlling plant diseases. Therefore, the current research was conducted with the aim of isolating and identifying endophytic fungi of beans with biological control potential. In this research, during the years 1400-1401, various tissues of disease-free bean plants were collected from bean farms in Fars province. After cutting plant tissues into small pieces and washing them with water, they were surface disinfected. For this purpose, they were immersed in 70% ethanol and then in 2% sodium hypochlorite solution for 1 and 2 min, respectively, followed by three rinses with sterile distilled water. After removing excess moisture from the plant pieces and cultivating them in PDA medium containing 200 mg/L of streptomycin sulfate, they were incubated at 28 °C for 7 to 14 days. Fungal isolates were purified by single spore method and were maintained on sterile filter paper. Based on the morphological characteristics on the OA, OMA, MEA, PCA, PA 1/10 and PDA culture media and with the help of 2019 keys, it was morphologically identified. They were also amplified and sequenced and molecularly identified using the ITS1/ITS4 and T1/TUB4Rd primers. Based on the morphological characteristics and phylogenetic analyses, Acrophialophora teleoafricana was identified as an endophytic fungus of bean root for the first time in the world.