شناسائی ویروس پیچیدگی برگ زرد گوجه‌فرنگی و بتاستلایت همراه در مزارع جعفری واقع در استان کرمان

Tomato yellow leaf curl virus (TYLCV) is an important species of the genus Begomovirus (family Geminiviridae). Members of the genus are transmitted by whiteflies (Bemisia tabaci) and caused destructive diseases in various crops in tropical and subtropical regions of the world. Association of three satellite molecules including alphasatellite, betasatellite and deltasatellite have been reported with the begomoviruses. To study geminiviruses and nanoviruses infecting leafy vegetables, a symptomatic parsley sample (K7) showing leaf curling, cup-shaped leaves and marginal white leaves was collected from leafy vegetable growing farms in Bagher-Abad (vicinity of Kerman, southeastern Iran). Total DNA was extracted from the sample using a C-TAB method followed by PCR assay using two degenerate and specific primer pairs and infection of sample with TYLCV and tomato yellow leaf curl betasatellite (ToLCB), respectively, was detected. However, the association of the alphasatellite molecule with the sample was not detected. Subsequently, the full-length genome of ToLCB was Sanger sequenced and used for phylogenetic analysis. Accordingly, the parsley isolate of ToLCB was grouped with other Iranian isolates of ToLCB available in GenBank. Previously, TYLCV and/or ToLCB infection of a number of crops, leafy vegetables including basil, Moldavian dragonhead, and weed species has been documented from agricultural farms in Kerman Province. To the best of our knowledge, it is the first report of parsley plant with TYLCV and ToLCB in the world. Full-length sequencing of the TYLCV genome (isolate K7), identification of the TYLCV strain and pathogenesis studies using constructs of the virus and the associated betasatellite are in progress.