بررسی ارتباط ژن های نشانگرجدیدبا مقاومت به پاتوسیستم پوسیدگی فوزاریومی ریشه و طبق پیاز

Fusarium basal rot (FBR) disease is a significant threat to onions globally. FBR-Fusarium basal rot (Fusarium oxysporum f. sp. cepae-FOC) is one of the most destructive onion diseases in the field and storage. Sources of resistance can provide a feasible method for management. However, knowledge on mechanisms of resistance is scarce. Thus, in this study, the selected populations of eighteen short-day onion genotypes for FOC susceptibility; genetic and transcriptome analysis using qRT-PCR with six novel selected marker genes: R1, R5, RGA29, lectin, LOX, and Osmotin, at tree time post inoculation (wpi) with 10 days’ interval were evaluated. Screening for resistance showed the lowest severity was in Saba – HS’ (4.7%) and ‘Saba’ (6.7%), respectively. It was also found that following inoculation with FOC could regulate defense-related marker genes in resistant onions '‘Saba’' and ‘Saba’-HS significantly. Marker genes, RGA29, R1 and PR5, were upregulated in the resistant genotypes by 3.83, 4.78 and 5.01-fold, respectively Surprisingly, Lectin, LOX, and Osmotin were also expressed to FOC simultaneous, though basically are resistance to biotic and abiotic stresses: Lectin to Rhizoctonia solani, Aphid, and major sap-sucking pests; LOX to root-knot and cyst nematode; Osmotin to drought stress, and oxidative burst in plants. In addition, there was also a significant increase in CAT, POX, and SOD enzyme activity in resistant genotypes in comparison to susceptible and non-inoculated controls. In conclusion, there is indication of double, and or multiple roles of selected marker genes covering two or more functions at a time. The findings introduce newly resistant onion genotypes, and also can be used in management programs against FOC. Cumulatively, the transcriptome-data provide novel-insights into the response of onions for improving onion-breeding to FOC.