مطالعه فیلوژنتیکی 28S rRNA برخی گونه های Laelapidae بر اساس Hypoaspis maryamae

The nuclear 28S rRNA gene (28S rDNA) has proved its usefulness on both deep and recent divergences, including mites. The family Laelapidae (Acari: Mesostigmata) is a diverse and varied group of mites which pose major problems in their taxonomic identification. In current research, the 28S rRNA sequences of Laelapidae family were retrieved from NCBI in FASTA format and 28S rRNA of Hypoaspis maryamae with accession number MH824151 which were identified in previous research in Iran added to the list. Due to the large number of sequences available in this family, analyzing all the sequences is not feasible. Consequently, one sequence from each species was selected as their representatives for phylogenetic analyses. Total data consisted of 60 laelapid species belonging to 25 genera. Phylogenetic analysis was conducted based on the nucleotide sequences using the Molecular Evolutionary Genetic Analysis; version 11.0.13 (MEGA 11.0.13 software) with the ClustalW algorithm, and the neighbor-joining (NJ) method with 1000 bootstrap replications. The results of current research showed that all 28s nucleotides were separated into five different parts, considered as Group I to Group V. All the species belonged to the groups except Andreacarus zumpti, Euandrolaelaps sp. and Hypoaspis rosei which were excluded from any groups. Groups III and V had 3 and 5 members, respectively, while four species categorized in groups II and IV. Group I was the biggest group, to which the other species belonged to this group (n=41). Hypoaspis maryamae classified in group I with a close relationship to Ichoronyssus miniopteri and Hymenolaelaps sp.. These outcomes along with the interpretations of current research suggested that, in species belonging to the Laelapidae family, 28S rRNAs are very similar to each other but have some differences which make this family complicated.