بررسی امکان وجود دیاپوز در شب پره مینوز گوجه فرنگی Tuta absoluta

Tomato leaf miner Tuta absoluta (Meyrick, 1917) (Lep. : Gelechiidae) is one of the most devastating pest of solanaceous plants worldwide, as it can reduce crop yield by up to 90% in both greenhouse and field conditions. Given the global distribution and high behavioral and physiological flexibility of T. absoluta, its diapause has been the subject of controversy. To investigate the potential conditions for diapause induction in T. absoluta, possible temperature and photoperiod conditions for diapause induction were employed from egg to adult emergence. To further the investigation, diapause - associated hormone levels were measured. In addition, the changes in the hormone content of cold - acclimated pupa were studied. The results showed the longest development time was observed in pupae reared at 15 ° C and 13 : 11 L : D (49.42 ± 0.8 days). However, at 18 and 25 ° C, despite the significant difference in the examined photoperiods compared to the control (16 hours of light and 8 hours of darkness), there were no considerable increase in the pupal period. In addition, Prothoracicotropic hormone (PTTH) and 20 - Hydroxyecdysone (20E) contents did not differ significantly between the pupae reared under long and short -day conditions. Furthermore, exposure to cold stress significantly decreased the concentrations of both hormones. Our research uncovered that low temperature is the primary factor limiting growth and activity in T. absoluta, and changes in hormone levels are linked to cold temperature. However, our studies have found no evidence of diapause in this insect.