برخی ویژگیهای بیوشیمیایی آنزیم پروتئاز و لیپاز در پروانه برگخوار شمشاد (Cydalima perspectalis Lep. Cerambidae) در شرایط آزمایشگاهی

Forest boxwood (Buxus sempervirens L., 1753) or Hyrcanian boxwood (Buxus hyrcana) is an evergreen tree native to the Hyrcanian forests in northern Iran, which is on the list of endangered plant species of the International Union for Conservation of Nature (IUCN: International Union for Conservation of Nature) contract. One of the most important pests of Hyrkani boxwood in Iran is the boxwood moth (Cydalima perspectalis, Lep. Cerambidae, Walker, 1859), which is not possible to use chemical pesticides against it due to the main place of activity of this insect (forest ecosystem). Researchers are looking for alternative methods for such pests. The digestive system and the enzymes of the insect body as a whole are known by many entomologists and disease vector experts as a potential target for controlling insects or controlling the transmission of disease-causing organisms. Meanwhile, two enzymes, lipase and protease, play a role in many biological processes in the body of insects. The purpose of this research was to investigate some biochemical characteristics of protease and lipase enzymes in boxwood moth. For this purpose, the digestive tract of four-year-old larvae was extracted and homogenized and total protease activity against azocasein and lipase enzyme against para-nitrophenylbutyrate as substrate in the temperature range of 20 to 37 degrees Celsius and acidity of 8.5, 9.5, 10.5 and 11.5, in laboratory conditions with the help of spectrophotometer in 440 and 405 nm wavelengths were investigated. The results showed that the highest proteolytic activity was at 10.5 acidity and the optimum temperature was 35°C. Optimum activity of lipase enzyme was seen at 37 degrees Celsius.