اولین گزارش از ویروس پالامپور پیچیدگی برگ گوجه‌فرنگی (ToLCPMV) در مزارع فلفل استان خوزستان

Tomato leaf curl Palampur virus (ToLCPMV) is an important virus with bipartite genome of genus Begomovirus and family Geminiviridae, which has spread in recent years in Iran, especially in southern regions. This virus was first reported from India and then from Pakistan, Iraq, Oman and Saudi Arabia. In order to detection this virus, sampling was carried out during the spring and summer seasons from pepper fields in the cities of Khouzestan province, including Dezful, Shoushtar and Andimeshk, which had a high population of Bemisia tabasi. DNA extraction by CTAB method and polymerase chain reaction (PCR) using PCRBio system kit and a pair of Begomvirus degenerate primers related to pre-coat protein gene (PCRv181/Bc) on DNA-A fragment carried out and resulted in the amplification of 550bp fragments in the samples collected from all three cities. These fragments were sequenced and evaluated using nBlast tool. Also, PCR assay using species-specific primers by amplifying a fragment of about 950bp of DNA-B including a part of the movement protein gene and the intergenic region, as well as determining the nucleotide sequence of the amplified region and comparing them with other sequences in the gene bank using nBlast tool confirmed the occurrence of ToLCPMV in the studied fields. Multiple sequence alignment of genomic sequences was done using ClustalW tool in Mega11 software and then a phylogeny tree was drawn by Neighbor joining method and based on 1000 repetitions in bootstrap evaluation. Also, the pairwise comparison matrix of the sequences and the analysis of the nucleotide sequence similarity of the studied genomic region between the sequenced isolates and the gene bank were performed using SDT v1.2 software. In the phylogenetic tree based on the nucleotide sequence of ToLCPMV movement protein gene region, isolates were classified into groups I and II. The results showed the sequenced isolates in this study, along with other Iranian isolates such as three isolates from Khouzestan province on watermelon and isolates from Kerman and Jiroft, were classified in group I. Iraq and Saudi Arabia Isolates were classified in this group alongside Iranian isolates. India and Pakistan isolates were separately placed in group II. According to the SDT software analyses, Khouzestan isolates had the similarity of 99.8% to each other. These isolates had the highest similarity (97.7%) and the least genetic distance with Iraq OP810507 and OP620406 isolates and Saudi Arabia OL416214 and ON843664 isolates. There was the lowest similarity between Khouzestan isolates and Indian isolate on eggplant (OM315020) with 89.1% similarity. This was the first report of ToLCPMV occurrence in pepper fields of Khouzestan Province.