بررسی اثر انتاگونیستی Lentinula edodes علیه گونه Alternaria dumosa بیمارگر مرکبات در شرایط آزمایشگاهی

Different species of the genus Alternaria cause several diseases as facultative pathogens in citrus trees. Various species of these fungi, including Alternaria alternata , Alternaria citri , Alternaria atra ,and Alternaria dumosa are pathogenic on different varieties of citrus in Iran. .Biologically active compounds, which are mainly synthesized by fungi , bacteria and plants can be used for various applications such as antifungal , antibacterial , and antiviral activity, resistance induction against stresses and plant growth promotion. Fungi in the Basidiomycota phylum can be considered as one of the most important producers of biologically active compounds. The Agaricales order is a group of fungi belonging to the Basidiomycota phylum, which are known for reasons such as the easy growth on different substrates and its important role in the environment, as well as high economic value. considering the severe damage of plant pathogens to strategic products and the harmfulness of using chemical compounds, the world community is expanding the use of biological compounds instead of chemical compounds in control of plant diseases. The aim of this research was to investigate antifungal effect of the non–volatile metabolite produced by Lentinula edodes in Potato Dextrose Broth medium and add it after 20 days with three concentrations of 0.1 , 1 , 10% to the Potato Dextrose Agar culture medium to observe its effect on the growth of Alternaria dumosa pathogenic on orange. Effect of the non–volatile fungal metabolite on growth of the A. dumosa isolate pathogenic on citrus was investigated after 8 days. At least 3 replications were used for each treatment in each round of performing the experiment and the assay was repeated three times. Comparison of the obtained results was done using Duncan’s multiple range test at p < 0.05level . Data evaluation revealed that among 3 concentrations tested, the non–volatile metabolites of lentinula edodes showed 46% inhibitory effect at 0.1% concentration as the highest mycelial growth inhibition of the fungal pathogen. Based on the findings of this research, the metabolites obtained from this edible and medicinal mushroom have considerable destructive effect on the Alternaria fungus pathogenic on citrus it is necessary to investigate their efficiency in control of the disease on the plant tissues in future research.