ارزیابی تاثیر تیمار بذر بر واکنش برخی ارقام گوجه فرنگی به ویروس پالامپور پیچیدگی برگ گوجه فرنگی

Tomato (Solanum lycopersicum L.) is one of the most important agricultural products in the world. According to the FAO statistics of 2021, tomatoes are cultivated on more than five million hectares in open fields and greenhouses, and their production yield is approximately 170 million tons annually. Tomato leaf curl Palampur virus (Begomovirus solanumpalampurense) causes the most persistent and severe losses of tomato crops. In this study, the effects of three tomato plants inoculated with tomato leaf curl Palampur virus were investigated to determine the effectiveness of bacterial, symbiotic fungal, and chemical agents for seed priming, damage reduction and plant growth stimulation under greenhouse conditions. The experiment was conducted at a temperature of 25±2 degrees Celsius and in a complete block randomized design with six replications. Treatments for each cultivar included inoculation with the virus (V), treated by Bacillus subtilis at concentrations of 108, 104, and 106 CFU/mL then the virus inoculation (BV); treated by beta-aminobutyric acid at concentrations of 2, 10 and 18 mM then the virus inoculation (BaV); and treated by Trichoderma harzianum at concentrations of 107, 1011, and 109 CFU/Ml then the virus inoculation (TV) and mock (C). The 3-leaf-stage plants were inoculated using the Agroinoculation method with ToLCPalV-A (JQ825226) and ToLCPalV-B (FJ660423) plasmids and symptoms severity was assessed at 31 days-post inoculation. Disease severity was measured using a scoring system from 0 to 4, with 4 indicating the highest infection rate. The analysis of variance revealed statistically significant differences at the 1% level in the reactions among cultivars. Among the treatments (TV, C2), the Money Maker treatment exhibited the lowest symptoms and disease severity, while the Tabas cultivar showed the highest disease severity. Further analysis of other investigated traits is in progress.