بررسی بیان تعدادی از ژنهای دخیل در مقاومت به بیماری پژمردگی فوزاریومی تحت تاثیر داروی رزوراترول

Induction of resistance in diseased plant using resistance inducers for reducing of chemical pesticide is goal of sustainable agriculture. In this study molecular change in infected tomato with fusarium wilting disease and treated with resveratrol drug was investigated. Fusrium oxysporum fsp. lycopersici isolate and tow tomato captain and termeh cultivars in a randomized block design with four repetition were used. Tow 100 and 150 µM concentration of resveratrol in 4-6 leaf stage were applied for this test. Tap water was used to irrigation and plant were monitored in greenhouse with controlled temperature and moisture. 100mg of fresh leaf of treated plants and non-treated plants (control) were used to RNA extraction and cDNA synthesis. qRT-PCR method using three NPR1,Eds and Pds genes was used to analysis of gene expression. Obtained data were subjected to analysis using ∆∆ct method. The results showed a significant different in expression level of tested genes in treated plants with resveratrol in compared to control plants. Although, the highest expression level was related to Npr1 gene in captain cultivar at 100 µM concentration of resveratrol drug and the lowest gene expression level was related to Pds gene in termeh cultivar at same concentration of resveratrol but between tow 100 and 150 µM concentration of resveratrol any significant expression level was showed. Based on these results, we could conclude that resveratrol is an active agent with high potential to induce resistance in plan against pathogen stress