اولین گزارش از وقوع ویروس پالامپور پیچیدگی برگ گوجه‌فرنگی (ToLCPMV) در مزارع لوبیا شهرستان الیگودرز

Tomato leaf curl Palampur virus (ToLCPMV) is a genus of Begomovirus and Geminiviridae family which has caused heavy damage to products in the southern provinces of the country in recent years. This virus is limited to phloem vessels and lacks mechanical transmission and the only agent that transmits them is the whitefly Bemisia tabaci. Due to the spread of the important and damaging ToLCPMV in the southern regions of the country and especially in the neighboring cities of Lorestan province, the purpose of this study is to detection ToLCPMV in the bean fields of Aligodarz county of Lorestan province which involves high population of B. tabaci. In order to detection and investigate the genetic diversity of ToLCPMV in the bean fields of Aligodarz county was sampled from suspected plants. In the PCR test, after using the specific degenerate primer pair of Begomoviruses transmitted by whiteflies, the fragments with an approximate size of 550bp were amplified which indicated Begomovirus infection in the investigated areas. In order to investigate more precisely, the sequence of the movement protein gene was amplified using a pair of specific primers to a size of approximately 950bp and the phylogeny tree was drawn with MEGA11 software and Neighbor-joining method. Also, the comparison matrix of pairs of nucleotide sequences of two isolates of Aligodarz bean with 20 selected isolates from the gene bank was drawn using SDT v1.2 software. Comparison of nucleotide sequences of Aligodarz isolates (Bean1.Lorestan and Bean2.Lorestan) in NCBI confirmed the detection of ToLCPMV in the studied area. Based on the analysis of nucleotide blast tool and phylogenetic tree, Bean1.Lorestan and Bean2.Lorestan Aligodarz isolates have the most similarity at the nucleotide sequence level to Andimeshk isolate OM831134 with 99.42% and Shoush isolate OM83113 with 98.30%. Also both isolates had the highest genetic distance and the least similarity to Indian isolate (OM315020) with 88.15%. Also, Aligodarz isolates showed averagely amino acid sequence similarity 96.30% and 98.15% with other sequences available in NCBI, respectively. The results of the two by two comparison of nucleotide sequences of Aligodarz isolate movement protein genomic region using SDT v1.2 software showed that the sequences of the two studied viral isolates are highly similar and their percentage of nucleotide similarity is 98.5% which were similar to the results of the phylogenetic tree. This was the first report of ToLCPMV widespread occurance in the bean fields of Aligodarz.