ارزیابی اثر ضد باکتریایی اسانس گیاه بومادران،Achillea millefolium ، بر روی گونه‌های مختلف باکتری های بیماریزای گیاهی

Achillea millefolium is an herbaceous perennial species from the Asteraceae family. This plant has upright stems and dense clusters of flowers. It thrives in sunny conditions and dry soil, making it suitable for withstanding extreme heat and dryness. A. millefolium has a substantial history of therapeutic usage across various cultures for treating wounds, fevers, and gastrointestinal ailments. It is purported to possess anti-inflammatory, antiseptic, and astringent properties. However, research on the antibacterial properties of the essential oil derived from A. millefolium is limited. The disk diffusion method is commonly employed to determine the antibacterial effects of different compounds against various bacterial strains. The effectiveness of these compounds can be assessed by measuring the zone of inhibition around essence-soaked discs. In this investigation, we have identified this botanical extract as a natural compound with significant antibacterial effects against various bacterial strains using the disk diffusion method. Initial assessment of the antimicrobial properties of the essential oil through the disk diffusion technique revealed inhibition zones ranging from 4.5 to 18 mm against the tested bacteria. Subsequent evaluation in broth media demonstrated activity against 9 bacteria, with minimum inhibitory concentrations ranging from 15mg ml-1. Notably, among the tested bacteria Burkholderia gladioli and Brenneria nigrifluens exhibited high and least sensitivity among plant pathogenic bacteria, respectively. These findings highlight the broad-spectrum antibacterial activity of A. millefolium essential oil, suggesting its potential application as a natural preservative for novel product development. Furthermore, the isolation of its bioactive constituents may offer opportunities for utilization in different agricultural sectors.