شناسائی میزبان‌های زینتی ویروس پیچیدگی برگ‌‌زرد گوجه‌فرنگی و بتاستلایت‌ همراه در جنوب استان کرمان

Tomato yellow leaf curl virus (TYLCV) (Begomovirus, Geminiviridae) is one of the most destructive begomoviruses infecting economic plants in the world. TYLCV has a wide distribution in south of Kerman province and has been reported from various host plants. During a survey, ornamental plants including zinnia and marigold showing yellowing, leaf curl, malformation and leaf distortion symptoms were collected from urban green space of Jiroft. Total DNA were extracted from samples and tested by PCR assay using degenerate primers of begomoviruses followed by PCR using specific primers which confirmed TYLCV infection of the samples. Circular DNA molecules of one positive sample from each plant species were enriched by phi29 DNA polymerase enzyme using commercial kit and rolling circle amplification (RCA) method. RCA products were digested with HindIII restriction enzyme and complete genome of the associated virus with the sample was cloned into the pGreen0029 plasmid and sequenced. Possible association of betasatellite molecule with the two samples (Zinnia-Jir 2 and Marigold-Jir4) were tested using PCR assay and specific overlapped primer pairs of ToLCB-F/ToLCB-R. The expected ~1.4 kb in size amplicon was cloned into the pTZ57R plasmid and Sanger sequenced. Analysis of the full sequences showed that the two ornamental plants are infected with tomato yellow leaf curl virus and tomato leaf curl betasatellite (ToLCB). Two TYLCV isolates shared maximum nucleotide identity with each other and with Iranian strains of TYLCV-OM from south of Kerman province which previously detected in soybean and broad bean. Two ToLCB isolates shared maximum nucleotide identity with other Iranian betasatellites isolates available in GenBank. To the best of our knowledge, this is the first report of infection of the ornamental plants including zinnia and marigold with TYLCV and the associated satellite (ToLCB) in the world. Considering the wide distribution of the virus in south of Kerman province, more studies about natural host range of the virus among other ornamental plants and the role of associated betasatellte molecule in aggravating the disease are in progress.