مطالعه ریخت‌شناسی، ریخت‌سنجی و مولکولیHemicycliophora poranga Monteiro and Lordello, 1978 (Nematoda: Criconematoidea) از استان مازندران

The genus Hemicycliophora, known as sheath nematodes, belongs to the Hemicycliophoridae family, has a long stylet, and is considered as an ectoparasitic nematode. To investigate the diversity of plant parasitic nematodes in the forests of Mazandaran province, in 2019 random sampling was done from the roots of the trees of Javarem forest park in Mazandaran province. Two populations of Hemicycliophora poranga species were recovered from rhizosphere of fig tree in Javarem park. Nematodes were extracted using sieving-centrifugation method. Permanent slides were prepared after fixing and transferring the nematodes to pure glycerin. The two populations' morphological and morphometric characteristics are consistent with their original description. This species has two different tail shapes, a conical tail with a blunt end and a cylindrical tail with a round end. In the three populations previously reported from Iran, only the first tail type has been reported. This research reports two populations with both types of tail shape and morphometric characteristics with minor differences (body length, total number of body annuli, stylet length, tail length and RV) from Iran. Also, to determine the phylogeny relationships of the studied species with its other isolates and species of the genus, the two 18S and 28S rDNA fragments of the recovered population were sequenced and Bayesian phylogenetic trees were reconstructed for both loci. This is the first report of the SSU sequence in the world. Morphological, morphometric and phylogeny characteristics of H. conida is the closest species to H. poranga.