بررسی روابط فیلوژنتیکی با استفاده از نشانگر پروتئینی Hsp70 در گونه های Bacillus subtilis و B. thuringiensis

Bacillus strains show their biological control capacity mainly through inhibitory activity on the growth of plant pathogens, as well as induction of systemic resistance in plants and competition for the ecological nich with plant pathogens. Hsp70 proteins, which are the result of dnaK gene translation, contribute to a wide range of protein folding processes in the cell by transiently connecting their substrate binding domain with short hydrophobic peptide segments in substrate proteins. After host infection, bacterial Hsp70 is in a position to initiate bacterial survival processes and stimulate the host's immune response. Any mutation in the dnaK gene has been shown to reduce bacterial viability within the host. In this study, amino acid sequences related to Hsp70 protein belonging to 41 isolates of Bacillus thuringiensis and 34 isolates of B. subtilis were extracted from UniProtKB site and clustered by version 11 of MEGA software. Understanding the phylogenetic relationships among strains of this bacterial genus is very important for the effective management of plant diseases and the development of targeted control strategies. After examining the drawn phylogeny tree, it was observed that this protein marker was highly accurate in distinguishing species, and these similarities and differences can be used to improve plant disease management processes. In order to confirm and further investigate these similarities and differences, the use of various molecular markers and phenotypic analysis of the isolates are needed in the future.