معرفی یک میزبان جدید برای دو گونه قارچی از تیره Didymellaceae در ایران

The plant Phlomis olivieri Benth., is a multi-purpose crop with high economic value worldwide and belongs to the Lamiaceae family. This plant is prevalent in Iran and other regions of the world and is used as a forage and medicinal plant. This study aimed to isolate and identify Didymellaceae species from various herbaceous plants in some provinces of Khuzestan, Ilam, and Chaharmahal and Bakhtiari. During the years 1400-1401, sampling was conducted in different seasons from Phlomis olivieri showing leaf spot symptoms. The samples were surface disinfected with 70% ethanol for one minute and 1-2 minutes with 1% sodium hypochlorite solution, followed by three times with sterile distilled water for one minute each. The samples were placed on potato dextrose agar (PDA) medium and incubated at 25 degrees Celsius for 7-14 days. Isolates belonging to the Didymellaceae family were selected for morphological and molecular studies. Fungal isolates were purified using single spore isolation method. Growth and morphological characteristics were examined on PDA medium. Portions of the tubulin gene (tub2) were amplified and sequenced using appropriate primers. The isolates were compared with known strains using BLASTn search algorithm and phylogenetic analyses. Based on morphological characteristics and phylogenetic analyses, the fungi Allophoma labilis and Epicoccum italicum were identified. These fungi are the first time that Phlomis olivieri has been reported in the world.