مطالعه ی ریخت‎شناسی و مولکولی گونه ناشناخته‎ای از جنسParatrichodorous Siddiqi 1973 (Nematoda:Trichodoridae) از استان کرمان

In order to identify plant-parasitic nematode of stone fruit trees in the west of Kerman province 40 soil samples in the year 2021 were collected from these orchards in the west of Kerman province. After extracting the nematodes from the soil by centrifugation and fixing and transferring them to glycerin, permanent slides were prepared from them. Among the isolated nematodes, an unknown species of Paratrichodorus genus was identified, which is proposed as a new member for the genus. This species has the highest frequency of males compared to females. Females of this species have a cylindrical and elongated body with 550 to 650 μm long, Onchiostyle 38 to 45 μm long, The esophagus overlaps ventrolaterally with the intestine a short vagina, approximately one-third of the body width in the area self, the female reproductive system has two ovaries and is stretched to both sides of the body, excretory pore 80 to 93 μm long, spicule curved ventrally with 38 to 45 μm long, The initial part of spicule to the last third of it has transverse grooves and a small bursa . Also, to determine the phylogeny relationships of the unknown species with other species of this genus and other genera of the Trichodoridae family, the the 28S rDNA D2-D3 region of the population was sequenced and the Baysian tree was reconstructed. Based on the molecular sequence results, this population is close to the species Paratrichodorus teres .In the resulting The studied population with a high posterior probability was placed in common clade to other populations of this genus.