جداسازی و شناسایی Beauveria pseudobassiana از شهرستان خرم‎آباد براساس ویژگی‎های مورفولوژی و مولکولی

Endophytic fungi (EF) can enhance both plant growth and defense barriers against pests and pathogens, contributing to the reduction of chemical pesticides and fertilizers use in agriculture. The genus Beauveria (Bals.) Vuill. is a entomopathogenic fungus with global distribution which recorded in various kinds of habitats and ecosystems including insects, soil and plants. Recent phylogenetic studies indicated that Beauveria showed a lot of genetic diversity and a complex that consists of a number of cryptic species. Currently, there are totally 21 species included in the genus. In this study, several Beauveria isolates were obtained from the soil samples collected from the Oak rhizosphere of Khorramabad county using a special culture media. After isolation and purification, identification was done based on a combination of morphological, morphometric and phylogenetic analysis with amplification of ITS-rDNA using ITS1 and ITS4 primers. Based on data obtained from the morphological observations viz. colony growth rate after 7 days at 25 ºC on PDA, (45 mm), the colony color on the PDA culture media, margin white with interior white color or changing to yellowish white color to pale yellow, Colony reverse uncolored or yellowish white, Odor indistinct, the diameter of the main hyphae (1-2 µm), phialides subspherical to ampulliform with 3–6 µm wide, conidia primarily subglobose or broadly ellipsoid, 2.2-2.5×2.5-2.7 µm, thin and smooth-walled. Also, comparing the sequence obtained with corresponding sequences in the GenBank, with 98-100% similarity and phylogenetic analysis, the identity of isolates was determined as Beauveria pseudobassiana.