ردیابی و تحلیل تبارزائی ویروس کوتولگی گوجه (Prune dwarf virus) از درختان گیلاس استان خراسان رضوی

Prune dwarf virus (PDV) from the genus Ilarvirus and the family Bromoviridae is a significant pathogen in cherry trees (Prunus avium L.) and causing serious damage to stone fruit trees. In spring 2022, samples showing symptoms including dwarfism, spotted fruits, decreased growth of buds and young shoots with short internodes in compared to healthy plants. were collected from cherry orchards in Golmakan, Khorasan Razavi Province. To detect PDV, a reverse transcription polymerase chain reaction assay was employed using specific primers targeting the movement protein of the virus. The amplified 756 in size nucleotide fragment of the Golmakan isolate was purified and Sanger sequenced. The obtained sequence was compared to available GenBank sequences using BLAST tool in NCBI database. Results indicated that the Golmakan isolate of PDV shared the highest nucleotide identity (99.7%) with the Bulgarian strain (MT152176), while the lowest identity (88.8%) was observed with the Netherlands strain (HM021231). A phylogenetic tree based on the movement protein was constructed, and the PDV strains were classified into two distinct groups, comprising the Golmakan isolate within the Group I. Furthermore, comparison of the movement protein sequences showed that amino acid sequences of the movement protein and the RNA-binding domain of the movement protein are highly conserved among the PDV isolates. In the current research, PDV was reported for the first time in Iran (GenBank accession OR541106), originating from a cherry garden and phylogenetic analysis of PDV was conducted, with a specific focus on the movement protein gene.