شناسایی مکان های ژنی مرتبط با مقاومت ارقام گندم به ویروس موزاییک نواری گندم با استفاده از روش نقشه یابی ارتباطی (Genome-wide association mapping)

Wheat streak mosaic disease with wheat streak mosaic virus agent is one of the important diseases of wheat. In order to identify quantitative trait loci (QTLs) for resistance to wheat streak mosaic virus in a population of 175 different wheat genotypes comprising Iranian cultivars and landraces from diverse world geographical origins, the genotypic data of 25066 DArTseq polymorphic markers (including 14339 Silico DArTs and 6484 SNPs) and also the phenotypic data of the reaction of the cultivars to wheat streak mosaic virus are used. The results of the phenotypic data showed that most of the cultivars were susceptible to wheat streak mosaic virus and only the cultivar IPK41079 was resistant to wheat streak mosaic virus. Based on studying the population structure the genotypes divided into three possible subpopulations. Genome-wide association study (GWAS) using the FarmCPU model identified 18 QTLs related to wheat streak mosaic virus resistance. The QTLs located on 13 chromosomes 1B, 2A, 2B, 2D, 3A, 3B, 4B, 5A, 5B, 6A, 6B, and 7A were mapped. The QTLs were physically mapped with the reference genome sequence of wheat. Three of the 18 QTLs were mapped with no protein coding sequences in the wheat reference genome sequence whereas, 15 QTLs with corresponding genes, including F-box proteins, proteins containing leucine-rich repeats, heat shock proteins 60 (HSP60), cytochrome P450, UDP-glucuronosyl /UDP-glucosyltransferase and serine-threonine/tyrosine-protein kinase were mapped. After confirmation and validation, the identified markers can be used in breeding programs to produce disease-resistant cultivars.