ز Phytoseiulus persimilis (Acari: Phytoseiidae) و Amblyseius swirskii های کنه شکارگری (Tetranychidae :Acari (urticae Tetranychus روی لوبیا ی تیمار شده با قارچ harzianum Trichoderma 6Tr در شرایط آزمایشگاهی

Soil-born microorganisms, including fungi, can affect the relation of plants and arthropods through interacting with roots, in the form of various mechanisms such as changes in plant defense mechanisms or the augmentation of absorption of nutrients. In this research, we studied the predation rate of P.persimilis Athias-Henriot and A.swiriskii Athias-Henriot using incubated bean plant infested with Tetranychus urticae Koch. Bean plants were treated with a suspension of Trichoderma harzianum Tr6 using the concentration of 10^8 (10 ml of suspension on each bean plant root) keeping in the greenhouse condition (25±5 ℃, 60% R.H. and 16L:8D hours). After 72 hours, detached bean leaves (3x3 cm2) placed upside down on water saturated cotton in 9 cm Petri dishes. Then three ovipositing T.urticae females were put on each patch for 48 hours. Just before starting the experiment, females and all the active stages of Tetranychus urticae were removed from patches and the number of eggs were equalized into 30 per patch. Same aged female predatory mites from either predator species were introduced into the patches individually, and after 8 hours, the number of eaten eggs was recorded. The experiment was performed in 10 replicates and data was analyzed using SPSS 25 software and independent T test. The results showed despite the significant difference in the oviposition rate of two-spotted spider mite on fungi treated bean plants in comparison with non-treated ones (p<0.01), the predation rate of A. swiriskii and P. persimilis on treated plants with the mentioned fungi did not show any significant differences (15.2±0.9, 17.2±2 and P>0.05). The results suggested that, treating the plants with Trichoderma harzianum Tr6 could not increase the predation rate of predators used here. The effect of the Trichoderma harzianum Tr6 on other behavioral and biological characteristics of these two predators would further be investigated