گزارش جدید از دو گونه‌ی Pestalotiopsis همراه با میوه درخت بلوط در استان ایلام

Oak is one of the most important trees in forest areas in Iran, especially Zagros. Ilam province with 641,665 hectares of forest is one of the most important habitats of Iranian oak (Quercus brantii). One of the most important issues on which the life and future of the forest depends, is the natural reproduction of the tree species in forest Seed production of Iranian oak species faces many problems due to destructive human activities in the forests as well as the presence of many pests and diseases, including those caused by fungi. In order to isolate and investigate the fungi associated with oak seeds, oak seeds were collected from different forest areas in Ilam province in 2021. The specimens were sterilized after washing with 96% alcohol then were sterilized in 5% sodium hypochlorite for 3 minutes and then were rinsed three times with sterilized distilled water, then cultured on PDA (potato-dextrose agar). For purification, the tip of hyphae method was used. After isolating fungal isolates from the endospore area of oak seeds, 17 fungal isolates from Pestalotiopsis genus were isolated. Identification of representative isolates were performed based on the combination of morphological characteristics and phylogeny based on the nucleotide sequence of ITS-rDNA and Part of the TEF gene. The results showed that, based on the available information, two species Pestalotiopsis chamaeropis and P. telopeae are reported for the first time for the Funga of Iran. Due to the fact that species of this genus have been reported as saprophytic, endophytic and plant pathogenic agents in the world on different plant species, pathogenicity tests and investigation of the effect of these species on the germination of oak seeds and their establishment in the soil are being carried out.