شناسایی گونه‌های ‌قارچی اندوفیت خانواده Chaetomiaceae در ارقام بومی گندم نان ایران

The species of Chaetomiaceae family have high diversity in terms of phenotypic and ecology so that species number of this family are increasing constantly through more investigation. On the other hand, most species of thermophilic fungi belong to Chaetomiaceae which are considered as a stable source of resistant enzymes against heat, from this point of view, they are important in biotechnology. They are also used in the pharmaceutical industry (anticancer cell lines, antimalarial and antituberculosis), Industrial (food, textile and fermentation industries) and Agriculture (biocontrol agents and biofertilizers). Therefore, the present study has been done to identify the fungal species of Chaetomiaceae from Iranian bread wheat landraces. For this purpose, plant roots, stems, leaves and pods were sterilized in 70% ethanol for 2 minutes, in 0.5% sodium hypochlorite with Tween 20 for 2 minutes, and 70% ethanol for 1 minute, and finally rinsed three times in sterile distilled water. After the growth of the fungal clonies on PDA, purification was done by hyphal tip method, and the isolates were cultured on OA, MEA, PCA medium for morphological assessment. Molecular characterization was carried out based on sequencing of genomic regions β-tubulin, RPB2 and ITS. According to morphological and molecular characterization, endophytic fungal species including Chaetomium grande, Ch. fimeti, Collarialla bostrychodes, Corynascus sepedonium and Botryotrichum mororum were Identified. Based on our knowledge, this is the first record of endophyte fungi of Chaetomiaceae in genotypes of Iranian bread wheat landraces.