واکنش ارقام مختلف گندم به ویروس موزاییک رگه‌ای گندم و ردیابی مولکولی ژن مقاومت Wsm2 در این ارقام با استفاده از نشانگر مولکولی

Wheat (Triticum aestivum L.) is one of the most important food products and as an important source of carbohydrate and vegetable protein in human food. One of the most destructive viral pathogens of wheat, which affects the yield and quality of this product, is the wheat streak mosaic virus (WSMV). Despite various agricultural and chemical control strategies, wheat streak mosaic virus (WSMV) is still a significant threat for yield and quality of wheat worldwide. It seems use of resistant varieties is one of the most effective methods for WSMV control. To evaluate reaction of the 287 different genotypes of wheat comprising 58 Iranian and 22 international varieties along with 207 other genotypes against WSMV, they were inoculated with WSMV-DOLT isolate collected from Isfahan at two-to-three-leaf stage. Symptom severity was assessed at 21, 28, and 35 days after inoculation (dpi) using a scoring scale ranging from 0 to 4. Scores ranged from plants showing no symptoms (score 0) to those exhibiting stripe mosaic and severe chlorosis (score 4). The results indicated that 15 genotypes were semi-resistant and six genotypes (five Iranian genotypes and one international genotype) showed no symptom and were classified as resistant at 35 dpi. Additionally, molecular detection of Wsm2 resistance gene using the Xbarc102 marker (SSR Marker- 219 bp), showed that the expected amplicon size (219 bp) was amplifies in all six resistant genotypes. This study provides valuable resources for breeders to improve resistant varieties against WSMV in wheat.