بررسی برهمکنش پروتئین – پروتئین معده میانیBemisia tabaci با انتقال ویروس پیچیدگی برگ پنبه (CLCuv)

The silverleaf whitefly (Bemisia tabaci) is one of several species of whitefly that are currently important agricultural pests. Its damage on plants includes removing plant sap, breakdown of the leaves of the plant, and leaf shedding. It is also a notorious vector for plant virus disease. Begomoviruses are a major group of plant viruses, transmitted exclusively by B. tabaci in a persistent circulative nonpropagative manner. This insect gut possesses some crucial protein receptors that allow specific entry of virus into the insect haemolymph. In this research, Interactions between two midgut proteins of B. tabaci which interact with CLCuV including thioredoxin-like protein (Accession no: AQM74407) and cytochrome oxidase subunit I (Accession no: AFQ62605) were predicted. STRING 12.0 database (http://string-db.org/) was used to predict the interacting proteins. The networks were created with a minimum required interaction score of 0.150 (low confidence), and the PPI lists were created. STRING is a database of known and predicted protein interactions. The database contains information from different sources, including experimental repositories, computational prediction methods and public text groups. According to the results, three KEGG enriched pathways were obtained by protein–protein interaction analysis which included: oxidative phosphorylation, citrate cycle (TCA cycle) and metabolic pathways. Additionally, various molecular functions were identified in these interactions, including NADH dehydrogenase (ubiquinone) activity, ubiquinol-cytochrome-c reductase activity, cytochrome-c oxidase activity, ubiquinone binding, electron transfer activity, and others. Alterations in these proteins can disrupt any of these cycles and interfere with the virus transmission process. This issue can be studied and examined as one of the methods to control the cotton whitefly and CLCuV virus.