عنوان مقاله: ردیابی و تحلیل تبارزائی سیتورابدویروس مرتبط با مرکبات (Citrus-associated rhabdovirus) از گیاه هندوانه‌ی ابوجهل در استان کرمان

The plant Citrullus colocynthis, belonging to the Cucurbitaceae family, is a medicinal herb native to the tropical regions of Iran, known for its use in treating various gastrointestinal diseases and diabetes. In the spring of 2021, samples were collected from the natural habitat of this plant in Jiroft area (Kerman province, Iran). The collected samples exhibited symptoms of yellowing, chlorosis, leaf curling, and stunted growth. After selecting samples with more severe symptoms, the total RNA of the plant was extracted and sent to Novogen (Beijing, China) for Next Generation Sequencing (NGS). Analysis of the raw data revealed a viral sequence with a length of 13,443 nucleotides, showing the highest similarity (80.82%) with the Citrus-associated rhabdovirus (MT302545) from the genus Cytorhabdovirus. A phylogenetic tree for this virus was constructed using the full-length genome and sequences of similar isolates available on the NCBI database. This sequence grouped with other CiaRV isolates, significantly diverging from other cytorhabdoviruses and forming a unique clade. All ORFs of this virus, numbered 1, 2, 3, 4, and 5, were identified and compared with the closest related strains. The nucleotide and amino acid identity between the identified isolate (CiaRV-IR-BA) and the closest strains were 80-90% and 85-95%, respectively, for almost all ORFs. This virus was identified for the first time in Iran, detected using RT-PCR, and registered with an accession number (OR232213) in the Gene bank.