شناسایی نماتدهای انگل گیاهان دارویی در استان گیلان

To identify plant parasitic nematodes of medicinal plants in Guilan province, 110 soil and root samples of medicinal plants were collected from the growing areas during the spring, summer and fall of the years 2022 and 2023. The nematodes were extracted, fixed and transferred to pure glycerin using common methods and permanent microscopic slides were prepared. The nematodes were evaluated using light microscope equipped with a digital camera and drawing tube. The nematodes were identified based on morphological and morphometric characters and using available identification keys. During this study, 18 species belonging to 13 genera including Aphelenchus avenae, Basiria graminophila, Coslenchus costatus, Crossonema civellae, Ditylenchus myceliophagus, Filenchus vulgaris, Helicotylenchus digonicus, H. exallus, H. vulgaris, Merlinius bavaricus, M. plerorbus, Mesocriconema xenoplax, Paratylenchus nanus, Pratylenchus neglectus, P. penetrans, P. thornei, Rotylenchus eximius and Zygotylenchus guevarai were identified. Among the identified species, A. avenae, H. vulgaris, and P. neglectus had the highest relative frequency percentage with 52%, 28%, and 17%, respectively. In this research, the species B. graminophila, C. costatus, C. civellae, D. myceliophagus, F. vulgaris, M. bavaricus, M. plerorbus, P. nanus, and R. eximius are reported for the first time from medicinal plants. The species Z. guevarai was extracted from the root tissue of oregano (Origanum vulgare) in Bandbon Deilaman. Due to the presence of the nematode in the root tissue, further investigations are being conducted on its parasitic behavior and damage.