کاربرد دو باکتری اندوفیت Mammaliicoccus sciuri و Chryseobacterium indologenes در مدیریت نماتد ریشه‌گرهی کیوی‌فروت در شرایط گلخانه

Root-knot nematodes (RKNs) are destructive plant pathogens that cause serious damages to kiwifruit cultivation. One of the safe methods for managing this group of nematodes is using of biological control agents. In this study, the assessment of the antagonistic ability of two endophytic bacteria Mammaliicoccus sciuri and Chryseobacterium indologenes against root-knot nematode, Meloidogyne incognita in the form of a completely randomized design with four treatments and five replications under greenhouse conditions were determined. Six months kiwifruit (Actinidia chinensis var. deliciosa (A. Chev) cv. Hayward) seedlings in plastic pots (2 kg) were treated with 40 ml bacteria suspension (108 CFU/ml). After two days, the seedlings were inoculated with 2000 second-stage juveniles (J2s) of M. incognita. Seedlings were maintained in the greenhouse under controlled conditions (25°C ± 2 and 70 % RH). After 50 days, the number of galls and egg masses, fresh weights of shoots and roots, J2s population of nematodes in the soil were evaluated. The results showed that both endophytic isolates significantly reduce the number of galls and egg masses on roots and J2s population in the soil and increase kiwifruit plant growth parameters, compared to the control. The reduction in the number of galls, egg masses and J2s population in the soil were 64.43, 76.39 and 81.36%, respectively in M. sciuri and 70.10, 81.15 and 89.28%, respectively in C. indologenes. Thus, these endophytic bacteria can be suitable candidates for microbial formulation to manage plant parasitic nematodes, improve plant growth by reducing nematode reproduction factors and increasing plant growth parameters.